Platelet-rich plasma (PRP) adalah plasma dengan konsentrasi trombosit yang tinggi, digunakan sebagai donor autologus faktor pertumbuhan bagi proses penyembuhan luka serta regenerasi jaringan. Berbagai metode preparasi PRP yang tersedia di pasaran berharga mahal. Metode Gama Double-Syringes merupakan metode murah dan sederhana preparasi PRP yang dikembangkan Bagian Ilmu Kesehatan Kulit dan Kelamin Fakultas Kedokteran Universitas Gadjah Mada (FK UGM).
     Sejak dikembangkan pada tahun 2008, belum pernah dilakukan pengujian terhadap nilai konsentrasi trombosit yang dihasilkan oleh metode ini. Tujuan dari penelitian ini adalah untuk mengetahui peningkatan kadar trombosit dan persentase platelet-loss pada preparasi Plasma Kaya Trombosit dengan Metode Gama Double-Syringes.
       Penelitian dilakukan dengan darah vena 5 subyek, masing-masing sebanyak 5 ml dan diukur kadar trombosit awalnya. Sediaan kemudian dipreparasi dengan Metode Gama Double- Syringes dan diukur kadar trombosit akhir serta persentase platelet-lost.
       Didapatkan hasil nilai mean kadar trombosit awal 223.000/μl dan nilai mean kadar trombosit akhir 474.000/μl sehingga terdapat peningkatan sebesar 2.15 kali lipat. Nilai mean platelet-loss didapatkan 17.4%.
       PRP hasil preparasi Metode Gama Double-Syringes menunjukkan peningkatan kadar trombosit rata-rata 2.15 kali lipat dengan platelet-loss rata-rata 17.4%, dan sudah menunjukkan keberhasilan klinis pada beberapa kasus.(MDVI 2011; 38/s: 21s - 23s)

Kata kunci : PRP, Gama Double-Syringes, trombosit, platelet-loss

       Platelet-rich Plasma is a highly concentrated thrombocyte preparation, use mostly in wound healing and tissue regeneration. Commercially PRP preparation methods are, as usual, expensive. Gama Double-Syringes is a simple and cheap method to produce Platelet rich-Plasma (PRP) developed by Department of Dermato-venereology, Medical Faculty of Medicine Gadjah Mada University. measurement of thrombocyte concentration produced by this method had been taken since its development in 2008, This research will measure the increase of thrombocyte concentration and platelet-loss of PRP produced by Gama Double-Syringes Method.
       From each of 5 subjects, 5 ml of venous blood is drawn and the baseline trombocyte concentration is measured. The final trombocyte concentration and platelet-loss were measured and calculated from the resulting PRP.
       The mean of baseline trombocyte concentrationis 223.000/μl while the mean of final,trombocyte concentration is 474.000/μl, or there is an increase of 2.15 fold. The mean of plateletloss is 17.4%.
     There is a 2.15 fold increase of trombocyte concentration with 17.4% platelet-loss in the PRP produced by Gama Double-Syringes Method. In our clinic, this locally-made PRP had shown good clinical result on several cases.(MDVI 2011; 38/s: 21s - 23s)

Keywords : PRP, Gama Double-Syringes, thrombocyte, platelet-loss